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# Chromogenic Endotoxin Quantification Kit for Accurate Bacterial Toxin Detection
## Introduction to Endotoxins and Their Detection
Endotoxins, also known as lipopolysaccharides (LPS), are toxic components found in the outer membrane of Gram-negative bacteria. These molecules can trigger severe immune responses in humans and animals, making their detection crucial in pharmaceutical, medical device, and biotechnology industries.
## The Importance of Accurate Endotoxin Measurement
Accurate quantification of endotoxins is essential for:
– Ensuring product safety in pharmaceutical manufacturing
– Validating medical device cleanliness
– Monitoring water quality in healthcare facilities
– Conducting microbiological research
## Features of Our Chromogenic Endotoxin Quant Kit
Our Chromogenic Endotoxin Quant Kit offers several advantages:
– High sensitivity with detection limits as low as 0.005 EU/mL
– Excellent linear range from 0.005 to 50 EU/mL
– Rapid results within 30-60 minutes
– Simple protocol requiring minimal sample preparation
– Compatibility with various sample types including water, biological fluids, and pharmaceutical products
## How the Chromogenic Assay Works
The kit utilizes a chromogenic substrate that reacts specifically with endotoxin-activated enzymes. The process involves:
1. Sample incubation with Limulus Amebocyte Lysate (LAL)
2. Activation of the coagulation cascade by endotoxins
3. Cleavage of the chromogenic substrate
4. Measurement of color development at 405-410 nm
## Applications Across Industries
### Pharmaceutical Manufacturing
Our kit is widely used for:
– Testing raw materials and finished products
– Validating cleaning procedures
– Monitoring production environments
### Medical Device Testing
Essential for ensuring device safety through:
– Detection of endotoxin contamination
– Validation of sterilization processes
– Quality control of implantable devices
### Research Laboratories
Valuable tool for:
– Studying bacterial pathogenesis
– Investigating host-pathogen interactions
– Developing new antimicrobial strategies
## Comparison with Other Detection Methods
Method | Sensitivity | Time Required | Complexity
Keyword: Chromogenic Endotoxin Quant Kit
Chromogenic | High (0.005 EU/mL) | 30-60 min | Moderate
Gel Clot | Moderate (0.03 EU/mL) | 60+ min | Simple
Turbidimetric | High (0.001 EU/mL) | 30-60 min | Complex
## Technical Specifications
Parameter | Specification
Detection Range | 0.005-50 EU/mL
Sample Volume | 50-100 μL
Incubation Time | 30-60 minutes
Storage Conditions | 2-8°C
Shelf Life | 12 months
## Why Choose Our Chromogenic Endotoxin Quant Kit?
Our kit stands out because of:
– Rigorous quality control standards
– Consistent lot-to-lot performance
– Comprehensive technical support
– Compliance with international pharmacopeia standards
– Cost-effective solutions for routine testing
## Ordering Information
For pricing and ordering details, please contact our technical support team or visit our website. We offer various package sizes to accommodate different testing volumes and requirements.